AP2A2 (E9Q1O) Rabbit mAb #55521

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	105
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

AP2A2 (E9Q1O) Rabbit mAb recognizes endogenous levels of total AP2A2 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro800 of human AP2A2 protein.

Background

The adaptor-protein 2 (AP-2) coat assembly protein complex is an important component of clathrin-coated pits involved in receptor-mediated endocytosis at the plasma membrane (1-3). Each AP-2 heterotetramer is composed of α, β, μ, and σ protein subunits. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into clathrin-coated vesicles destined for incorporation into the endosomal compartment. AP-2 complex subunit alpha-2 (AP2A2) is one of two large alpha-type adaptins that exist within the AP-2 complex. Research studies have shown that AP2A2 works in concert with AP-2 sigma subunits to facilitate the recognition of [ED]-X-X-X-L-[LI] endocytic motifs embedded within the cytoplasmic tails of membrane cargo proteins destined for the endosomal compartment (4).

Database Links

UniProt ID: O94973

Entrez-Gene Id: 161

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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