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Product Name

5088 Apaf-1 (R205) Antibody

8723 Apaf-1 (D7G4) Rabbit mAb

8969 Apaf-1 (D5C3) Rabbit mAb

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WB

Inquiry Info. # 4452

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Supporting Data

REACTIVITY	H M R Mk
SENSITIVITY	Endogenous
MW (kDa)	130, 140
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Apaf-1 antibody detects endogenous levels of human, mouse, and rat Apaf-1 as well as reported Apaf-1 splice varients ranging in molecular weight from 130 to 140 kilodaltons.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues within the central CED-4 homology domain of human Apaf-1. Antibodies are purified by protein A and affinity chromatography.

Background

Apoptotic protease activating factor 1 (Apaf-1), originally identified as the mammalian homolog of the C. elegans apoptotic regulatory protein CED-4, is an important signaling protein involved in the activation of caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms a complex with caspase-9 in the presence of cytochrome c and dATP, ultimately leading to caspase-9 activation and subsequent activation of caspase-3 (2,3). The protein contains an amino-terminal CARD domain, a central CED-4 homology domain, and multiple WD-40 repeats at the carboxy-terminus. Several isoforms of Apaf-1 are expressed through alternative splicing generating a small insert following the CARD domain as well as an extra WD-40 repeat (4). Apaf-1 knock-out mice display widespread defects in apoptosis and resistance to a variety of apoptotic stimuli (5,6).

Database Links

UniProt ID: O14727

Entrez-Gene Id: 317

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Apaf-1 Antibody #4452