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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

ApoE4 (4E4) Mouse mAb (BSA and Azide Free) #81462

Filter:
  • WB
  • ELISA
Western Blotting Image 1: ApoE4 (4E4) Mouse mAb (BSA and Azide Free)
Western blot analysis of extracts from HeLa cells, transfected with a construct expressing full-length human ApoE2 (hApoE2; +), ApoE3 (hApoE3; +), or ApoE4 (hApoE4; +), using ApoE4 (4E4) Mouse mAb (upper) or a pan ApoE antibody (lower). Data were generated using the standard formulation of this product.

To Purchase # 81462

Cat. # Size Qty. Price Ships
81462SF 100 µg
$843 Jan 22

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 35
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

This product is the carrier free version of product #8941. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #8941

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

ApoE4 (4E4) Mouse mAb (BSA and Azide Free) recognizes endogenous levels of total ApoE4 protein. This antibody does not cross-react with ApoE2 or ApoE3.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg130 of human ApoE4 protein.

Background

Apolipoproteins are plasma lipoproteins that function as transporters of lipids and cholesterol in the circulatory system. Chylomicrons are a fundamental class of apolipoproteins containing very low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) (1,2).

Human ApoE has three isoforms: ApoE2, ApoE3, and ApoE4. These three isoforms differ in the combination of cysteine and arginine residues located at positions 130 and 176. The ApoE4 isoform contains arginine at both locations. Research studies have linked ApoE4 function to neuronal plasticity, synaptogenesis, and neurodegenerative diseases (3). ApoE4 is produced in the liver and brain, although it is widely expressed in other tissues, such as the lung, spleen, and ovary. Investigators have established the ApoE4 allele as a genetic risk factor for Alzheimer’s disease (AD), accounting for 50-60% of the genetic variation in the disease (4). Research studies indicate that patients expressing ApoE4 have a reduced capacity for synaptic plasticity, an earlier age of onset of AD, and an increase in amyloid-beta (Aβ) deposition. The increase in Aβ suggests a role for ApoE4 in the impairment of amyloid clearance (5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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