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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Aprataxin (D5P3C) Rabbit mAb #52632

Filter:
  • WB
Western Blotting Image 1: Aprataxin (D5P3C) Rabbit mAb
Western blot analysis of extracts from various cell lines using Aprataxin (D5P3C) Rabbit mAb (upper) or β-Actin (D6F6) Rabbit mAb #8457 (lower).

To Purchase # 52632

Cat. # Size Qty. Price Ships
52632T 20 µl
$153 Jan 7
52632S 100 µl
$339 Jan 7

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 40
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Aprataxin (D5P3C) Rabbit mAb recognizes endogenous levels of total aprataxin protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe282 of human aprataxin protein.

Background

Aprataxin is a DNA repair protein that resolves DNA lesions (DNA adenylates) caused by abortive ligations in single-strand break repair, double-strand break repair and base excision repair (1). Aprataxin is recruited to sites of DNA damage by PARP1 (2). In the mitochondria, aprataxin and tyrosyl-DNA-phosphodiesterase 1 (TDP1) are required for repair of single strand breaks caused primarily by reactive oxygen species (ROS) (3).
The gene for aprataxin, APTX, is defective in the neurodegenerative disorder oculomotor apraxia type 1 (AOA1)(4). Researchers have shown that levels of aprataxin can predict patient response to irinotecan-based treatments in colorectal cancer (5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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