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Product last modified at: 2025-01-16T08:00:59.580Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

AQP4 (E4B6T) Mouse mAb #74806

Filter:
  • WB
  • IF
Western Blotting Image 1: AQP4 (E4B6T) Mouse mAb
Western blot analysis of extracts from various tissues using AQP4 (E4B6T) Mouse mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of AQP4 protein in rat liver tissue is consistent with the predicted expression pattern.

To Purchase # 74806

Cat. # Size Qty. Price Ships
74806T 20 µl
$152
74806S 100 µl
$336

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 28
Source/Isotype Mouse IgG2b kappa
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Frozen) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

AQP4 (E4B6T) Mouse mAb recognizes endogenous levels of total AQP4 protein. This antibody detects a 55 kDa band of unknown origin.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AQP4 protein.

Background

Aquaporins (AQPs) are integral membrane proteins that serve as channels to transfer water and small solutes across the membrane. There are 13 isoforms of AQP that are expressed in different types of cells and tissues (1,2). AQP1 is found in blood vessels, kidney, eye, and ear. AQP2 is found in the kidney, and it has been shown that the lack of AQP2 results in diabetes (1,3). AQP4 is present in the brain, where it is enriched in astrocytes (1,2,4). AQP5 is found in the salivary and lacrimal gland, AQP6 in intracellular vesicles in the kidney, AQP7 in adipocytes, AQP8 in kidney, testis, and liver, AQP9 is present in liver and leukocytes, and AQP10-11 in the intestine (1,3,4). AQPs are essential for the function of cells and organs. It has been shown that AQP1 and AQP4 regulate water homeostasis in astrocytes, preventing cerebral edema caused by solute imbalance (5). Several studies have shown the involvement of AQPs in the development of inflammatory processes, including innate and adaptive cell immunity (6,7).

AQP4 is expressed in brain cells, including neurons, but enriched in astrocytes, as well as in the peripheral nervous system (4,7). AQP4 influences synaptic plasticity, and the lack of this protein in the brain may cause memory and learning impairment via glutamate transporter 1 (GLT1) in Alzheimer's disease (8-10). It's suggested that AQP4 could play a role in the clearance of β-amyloid, and it may influence the transport of potassium and calcium in Alzheimer's disease (8,9). In Parkinson's disease, the lack of AQP4 results in an increased susceptibility of neurons from the substantia nigra and the ventral tegmental area for MPTP (11).
For Research Use Only. Not For Use In Diagnostic Procedures.
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