Render Target: STATIC
Render Timestamp: 2025-01-22T10:19:37.729Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-26 17:01:08.955
Product last modified at: 2025-01-01T09:02:57.033Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

AQP5 Antibody #59558

Filter:
  • WB
  • IP
Western Blotting Image 1: AQP5 Antibody
Western blot analysis of extracts from various cell lines and tissues using AQP5 Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of AQP5 protein in ZR-75-1 and SNU-1 cells, as well as in kidney, is consistent with predicted expression patterns.

To Purchase # 59558

Cat. #

Size

59558S
100 µl

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 22
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

AQP5 Antibody recognizes endogenous levels of total AQP5 protein. Based on the sequence of the immunogenic peptide, this antibody is not expected to cross-react with other aquaporin family members.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu262 of human AQP5 protein. Antibodies are purified by peptide affinity chromatography.

Background

Aquaporin 5 (AQP5) is a plasma membrane water channel whose overexpression in human cancers, such as breast, pancreatic, and colon cancers, has been associated with poor prognosis and metastasis. In colorectal cancer cells, AQP5 has been shown to regulate migration and invasion, as well as expression of epithelial-mesenchymal transition (EMT) factors. Knockdown of AQP5 inhibits the Wnt/β-Catenin pathway (1). Localization of AQP5 is altered in gastric cancer, and AQP5 is a marker for pyloric stem cells (2). In hepatocellular carcinoma (HCC), downregulation of AQP5 inhibits metastasis and EMT through inactivation of the NF-κB signaling pathway, and therefore, AQP5 is a potential therapeutic target for HCC (3). Phosphorylation of AQP5 at Ser156, possibly by PKA, activates the Ras signaling pathway in NIH/3T3 cells (4). AQP5 alters cell shape and actin dynamics without inducing EMT in normal epithelial Madin-Darby canine kidney (MDCK) cells, and this effect likely requires phosphorylation of AQP5 at Ser156 (5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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