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Render Timestamp: 2024-10-30T10:32:09.163Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-10-01 22:02:14.189
Product last modified at: 2024-10-05T08:00:16.738Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. AQP5 (F6X2V) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

AQP5 (F6X2V) Rabbit mAb #60340

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 22
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    AQP5 (F6X2V) Rabbit mAb recognizes endogenous levels of total AQP5 protein. Based on the sequence of the immunogenic peptide, this antibody is not expected to cross-react with other aquaporin family members. By western blot, this antibody may detect a band of unknown origin at approximately 12 kDa in some samples.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu262 of human AQP5 protein.

    Background

    Aquaporin 5 (AQP5) is a plasma membrane water channel whose overexpression in human cancers, such as breast, pancreatic, and colon cancers, has been associated with poor prognosis and metastasis. In colorectal cancer cells, AQP5 has been shown to regulate migration and invasion, as well as expression of epithelial-mesenchymal transition (EMT) factors. Knockdown of AQP5 inhibits the Wnt/β-Catenin pathway (1). Localization of AQP5 is altered in gastric cancer, and AQP5 is a marker for pyloric stem cells (2). In hepatocellular carcinoma (HCC), downregulation of AQP5 inhibits metastasis and EMT through inactivation of the NF-κB signaling pathway, and therefore, AQP5 is a potential therapeutic target for HCC (3). Phosphorylation of AQP5 at Ser156, possibly by PKA, activates the Ras signaling pathway in NIH/3T3 cells (4). AQP5 alters cell shape and actin dynamics without inducing EMT in normal epithelial Madin-Darby canine kidney (MDCK) cells, and this effect likely requires phosphorylation of AQP5 at Ser156 (5).

    Database Links

    UniProt ID: P55064


    Entrez-Gene Id: 362


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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