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Render Timestamp: 2024-11-22T10:39:19.462Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:56:16.482
Product last modified at: 2024-09-30T08:02:03.605Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Argonaute 4 (D10F10) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Argonaute 4 (D10F10) Rabbit mAb #6913

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 97
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Argonaute 4 (D10F10) Rabbit mAb recognizes endogenous levels of total Argonaute 4 protein. Experiments utilizing overexpression constructs indicated that this antibody may cross-react with Argonaute 1.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Argonaute 4 protein.

    Background

    Small non-coding RNAs are important regulators of gene expression in higher eukaryotes (1,2). Several classes of small RNAs, including short interfering RNAs (siRNAs) (3), microRNAs (miRNAs) (4), and Piwi-interacting RNAs (piRNAs) (5), have been identified. miRNAs are about 21 nucleotides in length and have been implicated in many cellular processes, such as development, differentiation, and stress response (1,2). miRNAs regulate gene expression by modulating mRNA translation or stability (2). miRNAs function together with the protein components in the complexes called micro-ribonucleoproteins (miRNPs) (2). Among the most important components in these complexes are Argonaute proteins (1,2). There are four members in the mammalian Argonaute family, and only Argonaute 2 (Ago2) possesses the Slicer endonuclease activity (1,2). Argonaute proteins participate in the various steps of miRNA-mediated gene silencing, such as repression of translation and mRNA turnover (1).

    Database Links

    UniProt ID: Q9HCK5


    Entrez-Gene Id: 192670


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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