ASF1B Antibody #2769
- WB
- IP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 19 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:25 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human ASF1B protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
ASF1 was first identified in S. cerevisiae based on its ability to de-repress transcriptional silencing when overexpressed (1). While only one gene exists in yeast and Drosophila, mammalian cells contain the two highly homologous ASF1A and ASF1B genes (2). ASF1A and ASF1B function as histone chaperones, delivering histone H3/H4 dimers to CAF-1 or HIRA histone deposition complexes to facilitate replication-coupled and replication-independent nucleosome assembly on DNA (2-5). Both ASF1A and ASF1B bind to CAF-1, but only ASF1A binds to HIRA (5). In addition to playing a role in DNA replication and gene silencing, ASF1 functions in DNA damage repair, genome stability and cellular senescence. Deletion of ASF1 in yeast and Drosophila confers sensitivity to various DNA damaging agents and inhibitors of DNA replication, increases genomic instability and sister chromatid exchange, and activates the DNA damage checkpoint (6-8). Depletion of both ASF1A and ASF1B in mammalian cells results in the accumulation of cells in S phase, increased phosphorylation of H2A.X, centrosome amplification and apoptosis (9,10). ASF1A is required for the formation of senescence-associated heterochromatin foci (SAHF), with overexpression of ASF1A inducing senescence in primary cells (4). Both ASF1A and ASF1B are phosphorylated in S phase by the Tousled-like kinases TLK1 and TLK2, and are dephosphorylated when TLK1 and TLK2 are inactivated by Chk1 kinase in response to replicative stress (11,12). The function of ASF1 phosphorylation is not yet understood.
- Singer, M.S. et al. (1998) Genetics 150, 613-632.
- Mousson, F. et al. (2007) Chromosoma 116, 79-93.
- Tang, Y. et al. (2006) Nat. Struct. Mol. Biol. 13, 921-929.
- Zhang, R. et al. (2005) Dev. Cell. 8, 19-30.
- Daganzo, S.M. et al. (2003) Curr. Biol. 13, 2148-2158.
- Ramey, C.J. et al. (2004) Mol. Cell. Biol. 24, 10313-10327.
- Prado, F. et al. (2004) EMBO Rep. 5, 497-502.
- Tyler, J.K. et al. (1999) Nature 402, 555-560.
- Sanematsu, F. et al. (2006) J. Biol. Chem. 281, 13817-13827.
- Groth, A. et al. (2005) Mol. Cell. 17, 301-311.
- Silljé, H.H. and Nigg, E.A. (2001) Curr. Biol. 11, 1068-1073.
- Carrera, P. et al. (2003) Genes Dev. 17, 2578-2590.
Limited Uses
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