ASS1 (D4O4B) XP® Rabbit mAb (BSA and Azide Free) #54846
- WB
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 47 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
For standard formulation of this product see product #70720
Storage
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Loss of ASS1 expression is one of the common metabolic alterations observed in many cancers, and it is a prognostic biomarker of reduced metastasis-free survival. ASS1 deficiency leads to the dependence of extracellular arginine for survival, proliferation, and cell growth. Ariginine starvation induces autophagy and apoptosis in ASS1 deficient cells and this has been exploited as a therapeutic intervention for the tumors with loss of ASS1 expression (4, 5). Pegylated arginine deiminase (ADI-PEG20), an enzyme that degrades arginine into citrulline, causes significant growth inhibition in tumors that have lost ASS1 expression, such as hepatocellular carcinoma, breast cancer, and sarcoma (6-8).
- Haines, R.J. et al. (2011) Int J Biochem Mol Biol 2, 8-23.
- Engel, K. et al. (2009) Hum Mutat 30, 300-7.
- Woo, H.I. et al. (2014) Clin Chim Acta 431, 1-8.
- Delage, B. et al. (2010) Int J Cancer 126, 2762-72.
- Feun, L. et al. (2008) Curr Pharm Des 14, 1049-57.
- Ensor, C.M. et al. (2002) Cancer Res 62, 5443-50.
- Qiu, F. et al. (2014) Sci Signal 7, ra31.
- Bean, G.R. et al. (2016) Cell Death Dis 7, e2406.
Limited Uses
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