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Product last modified at: 2025-01-01T09:03:22.715Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ASXL2 (E6Z3X) Rabbit mAb #71257

Filter:
  • WB
Western Blotting Image 1: ASXL2 (E6Z3X) Rabbit mAb
Western blot analysis of extracts from various cell lines using ASXL2 (E6Z3X) Rabbit mAb.

To Purchase # 71257

Cat. # Size Qty. Price Ships
71257T 20 µl
$153
71257S 100 µl
$339

Supporting Data

REACTIVITY H Mk
SENSITIVITY Endogenous
MW (kDa) 210
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ASXL2 (E6Z3X) Rabbit mAb recognizes endogenous levels of total ASXL2 protein.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala640 of human ASXL2 protein.

Background

Additional sex combs-like protein 2 (ASXL2) is a polycomb-associated protein that interacts with polycomb repressive complex 2 (PRC2), which contains the histone methyltransferase EZH2 and functions to mono-, di-, and tri-methylate histone H3 on lysine 27. These histone marks are associated with transcriptional repression (1). In addition, ASXL2 interacts with BRCA1-associated protein 1 (BAP1), the catalytic subunit of the polycomb repressive deubiquitinase complex (PR-DUB), which functions to de-ubiquitinate histone H2A at lysine 119 and activate transcription (2). ASXL2 functions as a transcriptional regulator of adipogenesis, acting to induce peroxisome proliferator-activated receptor gamma (PPARG) adipocyte differentiation, whereas its paralog ASXL1 functions to inhibit PPARG activity (3). ASXL2 is critical for animal viability and growth, as ASXL2 knockout mice experience partial embryonic lethality, premature death, axial skeletal abnormalities, and reduced bone mineral density (4,5). ASXL2 also plays a role in cancer, where ASXL2 truncation mutations are found in pancreatic, breast, and castration-resistant prostate cancers, albeit infrequently (6-9). ASXL2 is also frequently mutated in acute myeloid leukemia patients with t(8;21)/RUNX1-RUNX1T1 chromosomal translocations, and these mutations correlate with a 36% incidence of relapse (10).
For Research Use Only. Not For Use In Diagnostic Procedures.
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