Render Target: STATIC
Render Timestamp: 2025-01-22T13:52:20.143Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:57:22.146
Product last modified at: 2025-01-17T00:30:09.081Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

ATF-4 (E4Q4E) Mouse mAb #97038

Filter:
  • WB
  • IF
  • F
Western Blotting Image 1: ATF-4 (E4Q4E) Mouse mAb
Western blot analysis of extracts from 293T cells, vehicle-treated (-) or treated with Tunicamycin #12819 (2 μg/ml, 8 hr; +), and HeLa cells, vehicle-treated (-) or treated with Tunicamycin #12819 (2 μg/ml, 7 hr; +), using ATF-4 (E4Q4E) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 97038

Cat. # Size Qty. Price Ships
97038T 20 µl
$152
97038S 100 µl
$336

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 49
Source/Isotype Mouse IgG1 kappa
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Immunocytochemistry) 1:50 - 1:200
Flow Cytometry (Fixed/Permeabilized) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ATF-4 (E4Q4E) Mouse mAb recognizes endogenous levels of total ATF-4 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro134 of human ATF-4 protein.

Background

ATF-4, an activating transcription factor/cAMP-response element-binding protein family member, functions in the PERK and eIF2α ER stress responsive pathway (1-3). ER stress represses the translation of the majority of mRNAs, but selectively stimulates the translation of certain mRNAs including that of ATF-4 (2). Induced expression of ATF-4 increases the expression of genes critical for the recovery from ER stress (4).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.