Cell Signaling Technology Logo - Extra Large

Atg101 Antibody #8764

Filter:
  • WB

Inquiry Info. # 8764

Please see our recommended alternatives.

    Product Specifications

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 25
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Atg101 Antibody recognizes endogenous levels of total Atg101 protein. This antibody also detects a 55 kDa protein of unknown origin in some cell lines.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val177 of human Atg101 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Atg101 was discovered as a binding protein for Atg13, a component of the ULK1 serine-threonine kinase complex required for autophagy (1-3). Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (4,5). It is generally activated by conditions of nutrient deprivation, but is also associated with a number of physiological processes, including development, differentiation, neurodegeneration, infection, and cancer (6). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. The ULK1 complex includes both Atg13 and FIP200 and is required for starvation-induced autophagy (7-9). Interaction between Atg101 and Atg13 can be important for the stability and basal phosphorylation of Atg13 and ULK1 (1,2).

    Alternate Names

    ATG101; Atg13-interacting protein; ATGA1; autophagy related 101; Autophagy-related protein 101; C12orf44; chromosome 12 open reading frame 44; FLJ11773

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.