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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Atg101 (E1Z4W) Rabbit mAb #13492

Filter:
  • WB
Western blot analysis of extracts from various cell lines using Atg101 (E1Z4W) Rabbit mAb.

To Purchase # 13492

Cat. # Size Qty. Price
13492T 20 µl
$153
13492S 100 µl
$339

Product Specifications

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 25
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Atg101 (E1Z4W) Rabbit mAb recognizes endogenous levels of total Atg101 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val177 of human Atg101 protein.

Background

Atg101 was discovered as a binding protein for Atg13, a component of the ULK1 serine-threonine kinase complex required for autophagy (1-3). Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (4,5). It is generally activated by conditions of nutrient deprivation, but is also associated with a number of physiological processes, including development, differentiation, neurodegeneration, infection, and cancer (6). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. The ULK1 complex includes both Atg13 and FIP200 and is required for starvation-induced autophagy (7-9). Interaction between Atg101 and Atg13 can be important for the stability and basal phosphorylation of Atg13 and ULK1 (1,2).
For Research Use Only. Not for Use in Diagnostic Procedures.
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