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PDP - Template Name: Antibody Duet
PDP - Template ID: *******ad0fa02

PhosphoPlus® Atg16L1 (Ser278) Antibody Duet #10709

PhosphoPlus® Atg16L1 (Ser278) Antibody Duet: Image 1
Western blot analysis of extracts from AsPC-1, Calu-1, and HCT 116 cells, untreated (-) or treated with Torin 1 #14379 (250 nM, 2 hr; +) and/or the ULK1 inhibitor SBI-0206965 #29089 (50 μM, 2 hr; +), using Phospho-Atg16L1 (Ser278) (E7K6H) Rabbit mAb (upper), Atg16L1 (D6D5) Rabbit mAb #8089 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

To Purchase # 10709

Cat. # Size Qty. Price Ships
10709S 1 Kit
$706
  • Product Includes
  • Related Products
Product IncludesQuantityReactivityMW(kDa)Isotype
Phospho-Atg16L1 (Ser278) (E7K6H) Rabbit mAb #45511100 µlH M R68Rabbit IgG
Atg16L1 (D6D5) Rabbit mAb #8089100 µlH M R66, 68Rabbit IgG

Product Information

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been carefully selected to provide superior performance in specified applications.

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Control of autophagy was largely discovered in yeast and involves proteins encoded by a set of autophagy-related genes (Atg) (1). Formation of autophagic vesicles requires a pair of essential ubiquitin-like conjugation systems, Atg12-Atg5 and Atg8 (LC3)-phosphatidylethanolamine (LC3-PE), which are widely conserved in eukaryotes (2).Mammalian Atg16L1, containing an amino-terminal coiled-coil domain and carboxyl-terminal WD-repeats, has multiple isoforms produced by alternative splicing (3,4). Atg16L1 provides a functional link between the two crucial ubiquitin-like conjugation systems of autophagy. Atg16L1 binds Atg5 of the Atg12-Atg5 conjugate forming an 800 kDa multimeric complex (3). The Atg12-Atg-5-Atg16L1 complex localizes to pre-autophagosomal membranes, where it determines the site of LC3 lipidation and catalyzes the reaction required for the formation of mature autophagosomes (3,5). Genome-wide association scanning revealed variations in the Atg16L1 gene associated with Crohn's disease (6,7). Mice lacking the coiled-coil domain of Atg16L1 have impaired autophagosome formation and elevated inflammatory cytokines, consistent with its role in inflammatory disease pathogenesis (8). Hypomorphic Atg16L1 mice also show defects in autophagy and abnormalities in intestinal Paneth cell function similar to that found in Crohn's disease (9).

ULK1-mediated phosphorylation of Atg16L1 at Ser278 promotes xenophagy, a selective autophagic process directed against pathogens (10).

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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a registered trademark of Cell Signaling Technology, Inc.
KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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