Atg4A (D62C10) Rabbit mAb #7613
- WB
- IP
Supporting Data
| REACTIVITY | H R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 48-60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Atg4A predominately cleaves GATE-16, athough it can cleave the other mammalian Atg8 homologues with lesser efficiencies (4,7,8). Mutation in the corresponding Atg4A gene is critical for redox regulation and inhibits autophagosome formation. Expression of this Atg4A mutation demonstrates a role for reactive oxygen species in nutrient-deprived autophagy (9).
- Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
- Ohsumi, Y. (2001) Nat Rev Mol Cell Biol 2, 211-6.
- Kabeya, Y. et al. (2000) EMBO J 19, 5720-8.
- Kabeya, Y. et al. (2004) J Cell Sci 117, 2805-12.
- Mariño, G. et al. (2003) J Biol Chem 278, 3671-8.
- Sou, Y.S. et al. (2008) Mol Biol Cell 19, 4762-75.
- Scherz-Shouval, R. et al. (2003) J Biol Chem 278, 14053-8.
- Li, M. et al. (2011) J Biol Chem 286, 7327-38.
- Scherz-Shouval, R. et al. (2007) EMBO J 26, 1749-60.
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