Atg4B (D1G2R) Rabbit mAb #13507
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 48 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
While Atg4B displays a broad specificity for Atg8 homologues, it preferentially cleaves LC3 (7-9). Mutation in the corresponding Atg4B gene can be associated with strong inhibition of autophagosome formation. An excess of inactive Atg4B blocks lipidation of Atg8 homologues and inhibits autophagy. This makes Atg4B a potential tool for characterization of the isolation membrane and other autophagy studies (10,11).
- Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
- Ohsumi, Y. (2001) Nat Rev Mol Cell Biol 2, 211-6.
- Kabeya, Y. et al. (2000) EMBO J 19, 5720-8.
- Kabeya, Y. et al. (2004) J Cell Sci 117, 2805-12.
- Mariño, G. et al. (2003) J Biol Chem 278, 3671-8.
- Sou, Y.S. et al. (2008) Mol Biol Cell 19, 4762-75.
- Hemelaar, J. et al. (2003) J Biol Chem 278, 51841-50.
- Kabeya, Y. et al. (2004) J Cell Sci 117, 2805-12.
- Tanida, I. et al. (2004) J Biol Chem 279, 36268-76.
- Fujita, N. et al. (2008) Mol Biol Cell 19, 4651-9.
- Fujita, N. et al. (2009) Autophagy 5, 88-9.
Limited Uses
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