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ATP5A1 Antibody

ATP5A1 Antibody #18023

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	54
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ATP5A1 Antibody recognizes endogenous levels of total ATP5A1 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu209 of human ATP5A1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

ATP synthase alpha-subunit (ATP5A1) is a component of the mitochondrial complex V, which functions to generate ATP from ADP. Generation of ATP is catalyzed by ATP synthase. ATP synthase is composed of a soluble catalytic core, F₁, and a membrane-spanning proton channel, F₀. ATP5A1 is one of five different subunits that comprise the catalytic F₁ core. Interestingly, ATP5A1 is genetically linked to neurodevelopmental diseases (1). Moreover, the genetically linked mutant forms of C9ORF72, the most common genetic causes of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), bind to ATP5A1 and alter its protein expression, suggesting that mitochondrial dysfunction may be a major driver of disease in C9ORF72-linked ALS/FTD (2).

Database Links

UniProt ID: P25705

Entrez-Gene Id: 498

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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