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Render Timestamp: 2024-11-12T09:46:49.787Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:59:42.635
Product last modified at: 2024-09-30T08:01:12.054Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. ATP5B (E8A5A) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ATP5B (E8A5A) Rabbit mAb #85001

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 52
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ATP5B (E8A5A) Rabbit mAb recognizes endogenous levels of total ATP5B protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu523 of human ATP5B protein.

    Background

    ATP5B (ATP5F1B) is the catalytic β subunit of the F1 portion of the F1F0 ATP synthase, which is responsible for the majority of ATP synthesis in oxidative phosphorylation (1). ATP5B is present in the inner membrane of mitochondria in all types of cells, where it functions as an ATP provider and plays an important role in maintaining intracellular energy homeostasis. A high level of ATP5B is associated with cancer proliferation, metastasis, and drug resistance (2-4). An increased level of ATP5B plays an important protective role in decreasing renal fibrosis (5). Cell surface expression of ATP5B has been found in various cell types, including tumor, endothelial, and neuronal cells (6), where it is involved in multiple biological processes depending on the cell types. These include promoting viral entry (7), serving as a ligand for tumor-T cell recognition (8), generating extracellular ATP, contributing to intracellular PH homeostasis (9), and facilitating tumor metastasis (10). Inhibition of ATP5B has been proposed as a potential treatment for aggressive cancer (11,12).

    Database Links

    UniProt ID: P06576


    Entrez-Gene Id: 506


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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