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Render Timestamp: 2024-11-22T11:39:35.010Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:59:15.914
Product last modified at: 2024-11-18T13:00:17.683Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. ATP5O (E7F4U) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ATP5O (E7F4U) Rabbit mAb #92658

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 23
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ATP5O (E7F4U) Rabbit mAb recognizes endogenous levels of total ATP5O protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ATP5O protein.

    Background

    ATP synthase subunit O (ATP5O, OSCP, or ATP5PO) is a component of the mitochondrial complex V, which functions to generate ATP from ADP. ATP synthase is composed of a soluble catalytic core, F1, and a membrane-spanning proton (H+) channel, FO. ATP5O is part of the peripheral stalk linking the two structural domains. The ATP5O N-terminus directly interacts with subunit F1, while the C-terminus directly interacts with FO and is critical for the oligomycin sensitivity of the H+ channel (1,2). The expression of ATP5O is elevated in cancer-associated fibroblasts (CAFs) compared to normal fibroblasts in oral squamous cell carcinoma, reflecting the elevated oxidative phosphorylation requirements for ATP generation in these cells (3). Recent studies have shown elevated ATP5O as a biomarker for prostate and gastric cancers (4,5). Conversely, direct binding of claudin-10 (CLDN10) to ATP5O in the outer mitochondrial membrane increases ATP5O expression and acetylation, leading to disrupted mitochondria and reduced metastasis in clear cell renal cell carcinoma (ccRCC) (6).

    Database Links

    UniProt ID: P48047


    Entrez-Gene Id: 539


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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