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Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-03-07 13:08:37.465
Product last modified at: 2025-03-20T12:15:14.796Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Aurora B/AIM1 Antibody #3094

Filter:
  • WB
  • IP
  • F
Western Blotting Image 1: Aurora B/AIM1 Antibody
Western blot analysis of extracts from Hela, NIH/3T3 and C6 cells, untreated or nocodazole-treated (50 ng/ml, 24 hrs), using Aurora B/AIM1 Antibody.

To Purchase # 3094

Cat. # Size Qty. Price
3094T 20 µl
$131
3094S 100 µl
$306

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 40
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100
Flow Cytometry (Fixed/Permeabilized) 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Aurora B/AIM1 Antibody detects endogenous levels of Aurora B/AIM1 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human Aurora B/AIM1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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