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Render Timestamp: 2024-12-20T11:19:42.235Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-11-19 22:01:08.924
Product last modified at: 2024-05-30T07:04:08.745Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Autophagy Vesicle Elongation (Atg12 Conjugation) Antibody Sampler Kit

Autophagy Vesicle Elongation (Atg12 Conjugation) Antibody Sampler Kit #32345

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Autophagy Vesicle Elongation (Atg12 Conjugation) Antibody Sampler Kit provides an economical means of detecting proteins related to autophagy vesicle elongation pathway. The kit contains enough antibody to perform two western blot experiments per primary antibody.

    Specificity / Sensitivity

    Atg5 (D5F5U) Rabbit mAb recognizes endogenous levels of total Atg5 protein. This antibody is capable of detecting Atg5 conjugated to Atg12. Atg12 (D88H11) Rabbit mAb detects endogenous levels of total free and Atg5 bound Atg12 protein. Atg16L1 (D6D5) Rabbit mAb recognizes endogenous levels of total Atg16L1 protein. A background band is detected at 40 kDa in some cell lines. Atg7 (D12B11) Rabbit mAb recognizes endogenous levels of total Atg7 protein.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu265 of human Atg5 protein, a synthetic peptide corresponding to residues surrounding Ser36 of human Atg12 protein, a synthetic peptide corresponding to residues near the amino terminus of human Atg16L1 protein, and a synthetic peptide corresponding to residues near the amino terminus of human Atg7 protein.

    Background

    Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). Atg16L1 binds Atg5 of the Atg12-Atg5 conjugate forming an 800 kDa multimeric complex (9). The Atg12-Atg-5-Atg16L1 complex localizes to pre-autophagosomal membranes where it determines the site of LC3 lipidation and catalyzes the reaction required for the formation of mature autophagosomes (9,10).

    Database Links

    UniProt ID: Q676U5 , Q9H1Y0 , O94817 , O95352


    Entrez-Gene Id: 55054 , 9474 , 9140 , 10533


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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