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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Axin1 (C95H11) Rabbit mAb #2074

Filter:
  • WB
  • IP
  • IHC
Western Blotting Image 1: Axin1 (C95H11) Rabbit mAb
Western blot analysis of extracts from various cell lines using Axin1 (C95H11) Rabbit mAb.

To Purchase # 2074

Cat. # Size Qty. Price
2074T 20 µl
$152
2074S 100 µl
$336

Supporting Data

REACTIVITY H Mk
SENSITIVITY Endogenous
MW (kDa) 110
Source/Isotype Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunohistochemistry (Paraffin) 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #44419.

Protocol

Specificity / Sensitivity

Axin1 (C95H11) Rabbit mAb detects the endogenous levels of total Axin1 protein.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein of human Axin1.

Background

Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (1,2). Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling (3,4). In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) (5). Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice (6). Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop (7-9).

Pathways

Explore pathways related to this product.


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