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R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

β-Galactosidase (E2U2I) Rabbit mAb #27198

Filter:
  • WB
  • IF
Western Blotting Image 1: β-Galactosidase (E2U2I) Rabbit mAb
Western blot analysis of extracts from various cell lines using β-Galactosidase (E2U2I) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 27198

Cat. # Size Qty. Price
27198T 20 µl
$153
27198S 100 µl
$339

Supporting Data

REACTIVITY H M R Hm Mk
SENSITIVITY Endogenous
MW (kDa) 65
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Hm-Hamster 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Immunocytochemistry) 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

β-Galactosidase (E2U2I) Rabbit mAb recognizes endogenous levels of total β-galactosidase protein. The antibody cross-reacts with a 40 kDa protein of unknown identity in some cell extracts.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp39 of human β-galactosidase protein.

Background

β-galactosidase (also known as β-gal) is an essential hydrolase enzyme that catalyzes the hydrolysis of galactose-containing carbohydrates into monosaccharides. Substrates of β-galactosides include lactose, various glycoproteins, ganglioside GM1, and lactosylceramides. β-galactosidase is used widely in molecular biology; for example, isolation of recombinant bacteria during molecular cloning utilizes α-complementation of the bacterial β-galactosidase gene (lacZ) in the presence of a β-gal substrate to identify recombinant clones (1). In cell biology, Senescence-Associated beta-galactosidase (SA-β-gal), defined as β-gal activity at pH 6.0, is a widely used marker of replicative senescence. While initially thought to derive from a unique isoform of β-galactosidase expressed specifically in senescent cells (2), SA-β-gal activity was subsequently shown to result from overexpression and accumulation of β-galactosidase in endogenous lysosomes, and is not specifically required for replicative senescence (3).
For Research Use Only. Not For Use In Diagnostic Procedures.
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