β3-Tubulin (TU-20) Mouse mAb #4466
- WB
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Mouse IgG1 |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:100 - 1:400 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
β3-tubulin (TUBB3) is one of six β-tubulin isoforms and is expressed highly during fetal and postnatal development (axon guidance and maturation) (2). Its expression levels decrease in the adult central nervous system (CNS) but remain high in the peripheral nervous system (PNS) (3). Microtubules enriched in β3-tubulin are more dynamic than those composed of other β-tubulin isoforms (4). Research studies have shown that mutations in the β3-tubulin gene TUBB3 cause ocular motility defects and other nervous system disorders. Furthermore, β3-tubulin is present in neoplastic but not in normal differentiated glial cells. Thus, β3-tubulin is a great neuronal marker (5).
- Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.
- Jiang, Y.Q. and Oblinger, M.M. (1992) J Cell Sci 103 ( Pt 3), 643-51.
- Panda, D. et al. (1994) Proc Natl Acad Sci U S A 91, 11358-62.
- Tischfield, M.A. et al. (2010) Cell 140, 74-87.
- Katsetos, C.D. et al. (2003) J Child Neurol 18, 851-66; discussion 867.
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