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Render Timestamp: 2024-12-23T12:14:38.865Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-10-07 16:01:09.537
Product last modified at: 2024-05-31T15:15:07.755Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. BAF Complex IHC Antibody Sampler Kit

BAF Complex IHC Antibody Sampler Kit #93589

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The BAF Complex IHC Antibody Sampler Kit provides an economical means of detecting BAF complex members in formalin-fixed, paraffin-embedded tissue samples.

    Specificity / Sensitivity

    Each antibody in the BAF Complex IHC Antibody Sampler Kit detects endogenous levels of its target protein.

    Source / Purification

    Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Gly1293 of human ARID1A/BAF250A protein, Ala1320 of human ARID1B/BAF250B protein, Pro57 of human Brg1 protein, Gly264 of human BRM protein, Leu120 of human SMARCB1/BAF47 protein, Gln394 of human SS18 protein, and surrounding the fusion site of human SS18-SSX protein. Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PBRM1/BAF180 protein.

    Background

    The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histone-DNA contacts by ATP-dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP-dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM (1). This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant (1). SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle, and differentiation (1). Brg1/hBRM have been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors (2). Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression, including HDACs, Rb, and Tif1β (3-5). Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also plays a role as a tumor suppressor and Brg1 is mutated in several tumor cell lines (6-8).

    Database Links

    UniProt ID: A4PIV8 , Q15532 , P51531 , Q8NFD5 , O14497 , P51532 , Q12824 , Q86U86


    Entrez-Gene Id: 6760 , 6595 , 57492 , 8289 , 6597 , 6598 , 55193


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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