Render Target: STATIC
Render Timestamp: 2025-01-21T16:24:00.933Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:58:25.500
Product last modified at: 2025-01-02T19:15:08.396Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

BAG3 (E7Y9T) Rabbit mAb #23842

Filter:
  • WB
  • IP
  • IF
Western Blotting Image 1: BAG3 (E7Y9T) Rabbit mAb
Western blot analysis of extracts from various cell lines using BAG3 (E7Y9T) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of BAG3 protein in REH, SUP-B15, and Y79 cells is consistent with the predicted expression pattern.

To Purchase # 23842

Cat. # Size Qty. Price Ships
23842T 20 µl
$153 Jan 22
23842S 100 µl
$339 Jan 22

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 80
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200
Immunofluorescence (Immunocytochemistry) 1:400 - 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

BAG3 (E7Y9T) Rabbit mAb recognizes endogenous levels of total BAG3 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human BAG3 protein.

Background

BCL2-associated athanogene 3 (BAG3) is a member of the BAG domain-containing family of proteins important for protein homeostasis, including trafficking of misfolded proteins and the regulation of molecular chaperones, transcription factors, and autophagy (1-3). BAG3 is closely associated with molecular chaperones by interacting with the N-terminal ATPase domain of 70 kDa heat shock protein (HSP70) (4). HSP70 is a highly versatile molecular chaperone that is regulated by environmental stresses and interacts with unfolded proteins to promote proper refolding and prevent irreversible aggregation (5). BAG3 function has been associated with the regulation of apoptotic proteins and targeting polyubiquitinated client proteins for autophagic degradation (2). Expression of BAG3 is elevated in a variety of tumor types and regulates multiple aspects of cancer progression including cell adhesion, metastasis, angiogenesis, enhanced autophagic activity, and inhibition of apoptosis (2).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.