Bcl-3 (E4D4K) Rabbit mAb #16611
- WB
- IP
- IHC
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Bcl-3 (E4D4K) Rabbit mAb recognizes endogenous levels of total Bcl-3 protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human Bcl-3 protein.
Background
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
Bcl-3 is an atypical member of the IκB family. Unlike the classic IκB family members, Bcl-3 associates with p50/NF-κB1 or p52/NF-κB2 homodimers in nuclei and modulates transcription in a context-dependent manner (9,10). It is also involved in a recurring translocation, t(14;19)(q32;q13), found in some patients with B-cell chronic lymphocytic leukemia (B-CLL) and lymphoma (11,12).
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- Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
- Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
- Brown, K. et al. (1995) Science 267, 1485-8.
- Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.
- Schuster, M. et al. (2013) Cell Commun Signal 11, 23.
- Annemann, M. et al. (2016) Immunol Lett 171, 26-35.
- Michaux, L. et al. (1997) Cancer Genet Cytogenet 94, 36-43.
- Soma, L.A. et al. (2006) Hum Pathol 37, 218-30.
Limited Uses
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