Render Target: STATIC
Render Timestamp: 2024-11-21T13:34:05.337Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:58:01.855
Product last modified at: 2024-11-08T19:15:13.663Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

BLK (E8T1B) Rabbit mAb #66002

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 55
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    BLK (E8T1B) Rabbit mAb recognizes endogenous levels of total BLK protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro228 of human BLK protein.

    Background

    BLK is a Src family protein tyrosine kinase expressed in all stages of B cell development (1,2). Activation of B cells by various ligands is accompanied by activation of BLK (3). It has been suggested that BLK is involved in the control of B cell differentiation and proliferation (4,5). BLK transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that BLK may play an important role in thymopoiesis (6). BLK function may be redundant, however, as mice that do not express BLK are not impaired with respect to B cell development and immune response (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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