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Inquiry Info. # 4521

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Supporting Data

REACTIVITY	H M R Mk
SENSITIVITY	Endogenous
MW (kDa)	18
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Bok Antibody detects endogenous levels of total Bok protein. The antibody does not cross-react with endogenous levels of other Bcl-2 family members.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues within a central region of Bok. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Bok (Bcl-2-related ovarian killer)/ Mtd is a pro-apoptotic Bcl-2 family member identified in a yeast two-hybrid screen using Mcl-1 as the bait (1). Bok contains conserved Bcl-2 homology domains, BH1, BH2, and BH3. In yeast two-hybrid assays, Bok intereacted with some (Mcl-1, BHRF1, and Bfl-1) but not other (Bcl-2, Bcl-xL and Bcl-w) anti-apoptotic family members (1, 2). Likewise, Bok induced apoptosis is blocked by Mcl-1 and BHFR1 but not by Bcl-2 or Bcl-xL (1, 2). Bok was originally desribed to be predominately expressed in reproductive tissues such as ovary, testis and uterus (1). Subsequent studies have found selective expression of Bok in a number of adult and development tissues (2).

Database Links

UniProt ID: O35425

Entrez-Gene Id: 51800

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Bok Antibody #4521