Bora (D2B9) Rabbit mAb #12109
- WB
- IP
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Flow Cytometry (Fixed/Permeabilized) | 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Found to be conserved from C. elegans to humans, Bora is translocated from the nucleus to the cytoplasm upon activation of cdc2 at the onset of mitosis. Once present in the cytoplasm, Bora binds to and activates Aurora-A and PLK1 (3-5). It has been proposed that the binding of human Bora to PLK1 may lead to a conformational change in the protein that disrupts the autoinhibition by the Polo-Box Domain (PBD). This would allow for Thr210 on PLK1 to become more accessible for phosphorylation by Aurora-A (reviewed in 6). Active PLK1 then initiates the PLK1-cdc25-cdc2 positive feedback loop, leading to mitotic entry and the phosphorylation of Bora. Once phosphorylated in prophase, Bora is degraded allowing for normal mitotic progression (7).
- Nigg, E.A. (2001) Nat Rev Mol Cell Biol 2, 21-32.
- Archambault, V. and Carmena, M. (2012) Cell Cycle 11, 1490-5.
- Hutterer, A. et al. (2006) Dev Cell 11, 147-57.
- Seki, A. et al. (2008) Science 320, 1655-8.
- Chan, E.H. et al. (2008) Chromosoma 117, 457-69.
- Macurek, L. et al. (2009) Cancer Res 69, 4555-8.
- Seki, A. et al. (2008) J Cell Biol 181, 65-78.
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