Render Target: STATIC
Render Timestamp: 2024-11-22T11:09:33.018Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-11-18 16:03:11.248
Product last modified at: 2024-11-19T09:00:10.483Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

BORIS (F6R3W) Rabbit mAb #10806

Filter:
  • WB
  • IP
  • IF
  • F
  • ChIP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 90
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunofluorescence (Immunocytochemistry) 1:400 - 1:1600
    Flow Cytometry (Fixed/Permeabilized) 1:200 - 1:800
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    BORIS (F6R3W) Rabbit mAb recognizes endogenous levels of total BORIS protein. This antibody is predicted to detect isoforms 1, 7, and 8. This antibody detects a band of approximately 70 kDa that is predicted to be a BORIS isoform or cleavage product.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro629 of human BORIS protein.

    Background

    Brother of the Regulator of Imprinted Sites (BORIS), also known as CCCTC-binding factor like (CTCFL), is a DNA-binding protein that functions as a transcriptional modulator. Similar to CCCTC-binding factor (CTCF), BORIS plays critical roles in chromatin organization and is thought to impact the chromatin landscape by interfering with CTCF-mediated chromatin loops (1,2). BORIS can also act as a transcriptional coactivator by recruiting other activators and chromatin-modifying proteins (3-5). Unlike CTCF, which is expressed uniformly in both healthy and cancerous tissues, BORIS expression is normally restricted to the testis and embryonic stem cells (6,7). However, BORIS is aberrantly expressed in various types of cancer and it is associated with high-risk features that include resistance to treatment (8-11).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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