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Product last modified at: 2025-01-01T09:07:07.869Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

BORIS (F6R3W) Rabbit mAb #10806

Filter:
  • WB
  • IP
  • IF
  • F
  • ChIP
Western Blotting Image 1: BORIS (F6R3W) Rabbit mAb
Western blot analysis of extracts from K-562, OVCAR-8, and HT-29 cells using BORIS (F6R3W) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Low expression of BORIS protein in HT-29 cells is consistent with the predicted expression pattern.

To Purchase # 10806

Cat. # Size Qty. Price Ships
10806T 20 µl
$155
10806S 100 µl
$371

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 90
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
  • ChIP-Chromatin Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunoprecipitation 1:100
Immunofluorescence (Immunocytochemistry) 1:400 - 1:1600
Flow Cytometry (Fixed/Permeabilized) 1:200 - 1:800
Chromatin IP 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

BORIS (F6R3W) Rabbit mAb recognizes endogenous levels of total BORIS protein. This antibody is predicted to detect isoforms 1, 7, and 8. This antibody detects a band of approximately 70 kDa that is predicted to be a BORIS isoform or cleavage product.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro629 of human BORIS protein.

Background

Brother of the Regulator of Imprinted Sites (BORIS), also known as CCCTC-binding factor like (CTCFL), is a DNA-binding protein that functions as a transcriptional modulator. Similar to CCCTC-binding factor (CTCF), BORIS plays critical roles in chromatin organization and is thought to impact the chromatin landscape by interfering with CTCF-mediated chromatin loops (1,2). BORIS can also act as a transcriptional coactivator by recruiting other activators and chromatin-modifying proteins (3-5). Unlike CTCF, which is expressed uniformly in both healthy and cancerous tissues, BORIS expression is normally restricted to the testis and embryonic stem cells (6,7). However, BORIS is aberrantly expressed in various types of cancer and it is associated with high-risk features that include resistance to treatment (8-11).
For Research Use Only. Not For Use In Diagnostic Procedures.
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