Render Target: STATIC
Render Timestamp: 2025-03-10T10:04:49.553Z
Commit: a619ae74f66dae0f27639e88da12bcf600e46428
XML generation date: 2025-03-07 13:07:49.487
Product last modified at: 2024-05-30T07:08:03.460Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

BRF1/2 Antibody #2119

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  • WB

Inquiry Info. # 2119

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    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 40 to 50, 62
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    This antibody detects endogenous levels of total BRF1 and BRF2 proteins.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Chicken, Bovine

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human BRF1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    ZFP36L1, also known as butyrate response factor-1 (BRF1), and ZFP36L2, also known as butyrate response factor-2 (BRF2), both belong to the TIS11 family of CCCH zinc-finger proteins (1). This family of proteins, which also includes tristetraprolin (TTP), bind to AU-rich elements (AREs) found in the 3'-untranslated regions of mRNAs and promote deadenylation and rapid degradation by the exosome (2,3). These proteins play a critical role in cell growth control by regulating the mRNA turnover of multiple cytokines, growth factors, and cell cycle regulators, including GM-CSF, TNFα, IL-2, IL-3, and IL-6 (4,5). Deregulated ARE-mRNA stability can contribute to both inflammation and oncogenic transformation (6-8). Insulin-induced stabilization of ARE-containing transcripts is mediated by Akt/PKB phosphorylation of ZFP36L1 at Ser92, which results in binding by 14-3-3 protein and inactivation of ZFP36L1 (9). ZFP36L1 and L2 have also been shown to promote cell quiescence in developing B lymphocytes, promoting VDJ recombination (10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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