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Product last modified at: 2025-01-12T18:00:08.561Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Brg1 (E8V5B) Mouse mAb #72182

Filter:
  • WB
  • IHC
Western Blotting Image 1: Brg1 (E8V5B) Mouse mAb
Western blot analysis of extracts from various cell lines using Brg1 (E8V5B) Mouse mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

To Purchase # 72182

Cat. # Size Qty. Price Ships
72182T 20 µl
$150
72182S 100 µl
$350

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 220
Source/Isotype Mouse IgG1
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #98822.

Protocol

Specificity / Sensitivity

Brg1 (E8V5B) Mouse mAb recognizes endogenous levels of total Brg1 protein. This antibody does not cross-react with BRM protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro57 of human Brg1 protein.

Background

The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histone-DNA contacts by ATP-dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP-dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM (1). This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant (1). SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle, and differentiation (1). Brg1/hBRM have been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors (2). Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression, including HDACs, Rb, and Tif1β (3-5). Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also plays a role as a tumor suppressor and Brg1 is mutated in several tumor cell lines (6-8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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