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Commit: 642d75590e907c0f7dfc7c6e3b846bcc0b02197c
XML generation date: 2024-08-01 15:30:54.231
Product last modified at: 2024-08-29T20:15:08.075Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. BST2 (E4N3W) XP® Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

BST2 (E4N3W) XP® Rabbit mAb #95940

Filter:
  • WB
  • IHC
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 28-40, 50-70
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    IHC Leica Bond 1:400 - 1:1600
    Immunohistochemistry (Paraffin) 1:200 - 1:800
    Flow Cytometry (Live) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #38142.

    Protocol

    Specificity / Sensitivity

    BST2 (E4N3W) XP® Rabbit mAb recognizes endogenous levels of total BST2 protein. This clone shows a preference for the BST2 dimer by western blot.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with human BST2 recombinant protein.

    Background

    BST2 (CD317, Tetherin, HM1.24) is a type II transmembrane glycoprotein functioning as a major mediator of the innate immune defense against the dissemination of enveloped viruses by tethering virion on the cell surface (1). BST2 has an N-terminal cytoplasmic tail for endocytosis and cytoskeletal signaling, a transmembrane domain, an extracellular domain containing putative disulfide bonds and coiled-coil region for homodimer formation, and a C-terminal GPI domain for membrane anchoring (2,3). Both the transmembrane domain and the GPI domain can insert either into the cell membrane or the viral envelope membrane and hold them together to prevent viral release. Some viruses encode proteins, such as HIV-1 and Vpu, respectively, to act as antagonists to counteract BST2 (2,3). BST2 is overexpressed in gastrointestinal cancers, breast cancer, lung cancer, and multiple myeloma (4-7). BST2 monoclonal antibody targeting myeloma or lung cancer cells induces cellular cytotoxicity and cell death (ADCC, antibody-dependent cell-mediated cytotoxicity). Thus, BST2 serves as a potential target for tumor immunotherapy.

    Database Links

    UniProt ID: Q10589


    Entrez-Gene Id: 684


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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