Render Target: STATIC
Render Timestamp: 2025-01-21T11:15:50.785Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-08-01 15:28:38.402
Product last modified at: 2025-01-01T09:05:24.630Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

C/EBPβ Antibody #3082

Filter:
  • WB
Western Blotting Image 1: C/EBPβ Antibody
Western blot analysis of extracts from untreated or serum treated PC12 cells, using C/EBPbeta Antibody.

To Purchase # 3082

Cat. # Size Qty. Price Ships
3082S 100 µl
$306

Supporting Data

REACTIVITY R
SENSITIVITY Endogenous
MW (kDa) 38, 41
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

C/EBPbeta Antibody detects endogenous levels of rat C/EBPbeta protein. It does not cross-react with other C/EBP family members such as C/EBPalpha, -delta, -gamma, -epsilon or -zeta. It also does not recognize the p20 LIP rat C/EBPbeta isoform.

Species Reactivity:

Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser105 of rat C/EBPbeta. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors critical for cellular differentiation, terminal functions, and inflammatory response (1). Six members of the family have been characterized (C/EBPα, -β, -γ, -δ, -ε, and -ζ) and are distributed in a variety of tissues (1). There are two forms of C/EBPβ, the 38 kDa liver activating protein (LAP) and the 20 kDa liver inhibitory protein (LIP) which may be products of alternative translation. The 38 kDa LAP protein is a transcriptional activator while LIP may act as an inhibitor of C/EBPβ transcriptional activity (2). Phosphorylation of C/EBPβ at distinct sites stimulates its transcriptional activity (3-5). Phosphorylation at serine 105 of rat C/EBPβ, a unique site only present in the rat sequence, seems essential for rat C/EBPβ activation (6).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.