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  1. Products /
  2. Primary Antibodies /
  3. c-Maf (E2Q5D) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

c-Maf (E2Q5D) Rabbit mAb #26745

Filter:
  • WB
  • IP
  • IF
  • F
  • ChIP
Western Blotting Image 1: c-Maf (E2Q5D) Rabbit mAb
Western blot analysis of extracts from various cell lines using c-Maf (E2Q5D) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative expression of c-Maf protein in HeLa, A-172, and Jurkat cells is consistent with the predicted expression pattern.
1/6

To Purchase # 26745

Cat. # Size Qty. Price
26745T 20 µl
$156
26745S 100 µl
$371

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 50, 70
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
  • ChIP-Chromatin Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products
  • Conjugates

Product Information

Product Usage Information

For optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100
Immunofluorescence (Immunocytochemistry) 1:200 - 1:800
Flow Cytometry (Fixed/Permeabilized) 1:400 - 1:1600
Chromatin IP 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

c-Maf (E2Q5D) Rabbit mAb recognizes endogenous levels of total c-Maf protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human c-Maf protein.

Background

Transcription factor c-Maf, also known as MAF and V-maf musculoaponeurotic fibrosarcoma oncogene homolog, is a basic leucine zipper transcription factor (bZIP) in the large Maf protein subclass and belongs to the AP-1 superfamily (1). In humans, there are three c-Maf isoforms and in mice, only two isoforms have been characterized (2). Maf proteins contain a highly conserved ancillary DNA binding domain, N-terminal to the basic domain, and bind to relatively long DNA sequences termed Maf-recognition elements (MAREs) (3). c-Maf can form homodimers and heterodimers with other bZIP proteins like Jun and Fos, as well as non-bZIP proteins (4,5). c-Maf plays an important role in lens fiber cell development, bone development, apoptosis, and the immune system (6,7). In T cells, c-Maf expression is induced by TCR stimulation, and sustained by co-stimulatory signals, and/or cytokines like IL-4, IL-6, TGF-β, and IL-27 (8-12). In macrophages, c-Maf is required for self-renewal and promotes IL-10 production while inhibiting IL-12 (13,14). c-Maf plays a critical role in intestinal immune homeostasis, regulating ILC3s, Th17 cells, γδ T cells, and regulatory T (Treg) cells (7). c-Maf is an oncogene that contributes to the progression of some hematological malignancies and is expressed in other cancers but is not always correlated with poor prognosis (15,16). 
  1. Nishizawa, M. et al. (1989) Proc Natl Acad Sci USA 86, 7711-5.
  2. Imbratta, C. et al. (2020) Front Immunol 11, 206.
  3. Kataoka, K. et al. (1994) Mol Cell Biol 14, 700-12.
  4. Kerppola, T.K. and Curran, T. (1994) Oncogene 9, 675-84.
  5. Huang, W. et al. (2002) J Biol Chem 277, 50668-75.
  6. Kawauchi, S. et al. (1999) J Biol Chem 274, 19254-60.
  7. Cosovanu, C. et al. (2022) J Exp Med 219, e20220233. doi: 10.1084/jem.20220233.
  8. Bauquet, A.T. et al. (2009) Nat Immunol 10, 167-75.
  9. Kurata, H. et al. (1999) Immunity 11, 677-88.
  10. Yang, Y. et al. (2005) J Immunol 174, 2720-9.
  11. Xu, J. et al. (2009) J Immunol 182, 6226-36.
  12. Apetoh, L. et al. (2010) Nat Immunol 11, 854-61.
  13. Soucie, E.L. et al. (2016) Science 351, aad5510.
  14. Cao, S. et al. (2002) J Immunol 169, 5715-25.
  15. Morito, N. et al. (2006) Cancer Res 66, 812-9.
  16. Uhlen, M. et al. (2017) Science 357, eaan2507. doi: 10.1126/science.aan2507.

Database Links

UniProt ID: O75444


Entrez-Gene Id: 4094


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