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Product last modified at: 2025-01-01T09:04:37.245Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CA12 (D75C6) XP® Rabbit mAb #5864

Filter:
  • WB
  • IP
  • F
Western Blotting Image 1: CA12 (D75C6) XP® Rabbit mAb
Western blot analysis of extracts from SK-OV-3 and MCF7 cells using CA12 (D75C6) XP® Rabbit mAb.

To Purchase # 5864

Cat. # Size Qty. Price Ships
5864T 20 µl
$168
5864S 100 µl
$371

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 45-50
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200
Flow Cytometry (Live) 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CA12 (D75C6) XP® Rabbit mAb detects endogenous levels of total CA12 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly39 of human CA12 protein.

Background

Carbonic anhydrases (CA) are a family of ancient zinc metalloenzymes found in almost all living organisms. All CA can be divided into 3 distinct classes (α, β, and γ) that evolved independently and have no significant homology in sequence and overall folding. All functional CA catalyze the reversible hydration of CO2 into HCO3- and H+ and contain a zinc atom in the active sites essential for catalysis. There are many isoforms of CA in mammals and they all belong to the α class (1,2).
CA12, a member of the α class, is a plasma membrane protein with the catalytic domain in the extracellular space. CA12 is widely expressed in many tissues and is found at high levels in renal cell cancers. Its expression is induced by hypoxia in solid tumors and is considered to be a tumor marker (3-5). Genetic mutations in CA12 are associated with hyperchlorhidrosis (6,7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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