Render Target: STATIC
Render Timestamp: 2024-12-13T10:40:33.225Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-20 06:15:40.075
Product last modified at: 2024-09-13T07:01:13.034Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Calbindin Antibody #2136

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Inquiry Info. # 2136

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    Supporting Data

    REACTIVITY H M R Dm
    SENSITIVITY Endogenous
    MW (kDa) 28
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Dm-D. melanogaster 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:200
    Immunofluorescence (Frozen) 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Calbindin Antibody detects endogenous levels of total calbindin protein.

    Species Reactivity:

    Human, Mouse, Rat, D. melanogaster

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 200 of human calbindin. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Calcium-binding proteins of different subfamilies regulate the second messenger calcium. Calbindin, calmodulin, S-100, parvalbumin, and troponin C are members of the low molecular weight calcium-binding protein family (1). Calbindin is expressed in discrete neuronal populations within the CNS and is thought to act as an intracellular calcium buffering protein. Most Purkinje cells express calbindin, which is expressed when neurons start to migrate and differentiate. In contrast, other calcium buffering proteins, such as parvalbumin, are expressed later during development and in parallel with increasing neuronal activity (2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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