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Product Name

2173 Calbindin (C26D12) Rabbit mAb

Filter:

WB
IHC
IF

Inquiry Info. # 2136

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Supporting Data

REACTIVITY	H M R Dm
SENSITIVITY	Endogenous
MW (kDa)	28
SOURCE	Rabbit

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry
IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Dm-D. melanogaster

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:200
Immunofluorescence (Frozen)	1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Calbindin Antibody detects endogenous levels of total calbindin protein.

Species Reactivity:

Human, Mouse, Rat, D. melanogaster

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 200 of human calbindin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Calcium-binding proteins of different subfamilies regulate the second messenger calcium. Calbindin, calmodulin, S-100, parvalbumin, and troponin C are members of the low molecular weight calcium-binding protein family (1). Calbindin is expressed in discrete neuronal populations within the CNS and is thought to act as an intracellular calcium buffering protein. Most Purkinje cells express calbindin, which is expressed when neurons start to migrate and differentiate. In contrast, other calcium buffering proteins, such as parvalbumin, are expressed later during development and in parallel with increasing neuronal activity (2).

Database Links

UniProt ID: P05937

Entrez-Gene Id: 793

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Calbindin Antibody #2136