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R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Calbindin (D1I4Q) XP® Rabbit mAb #13176

Filter:
  • WB
  • IP
  • IHC
  • IF
Western Blotting Image 1: Calbindin (D1I4Q) XP® Rabbit mAb
Western blot analysis of extracts from various cell lines using Calbindin (D1I4Q) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 13176

Cat. # Size Qty. Price
13176T 20 µl
$155
13176S 100 µl
$371

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 28
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products
  • Conjugates

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunohistochemistry (Paraffin) 1:800
Immunofluorescence (Frozen) 1:200
Immunofluorescence (Immunocytochemistry) 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier-free (BSA and azide free) version of this product see product #46343

Protocol

Specificity / Sensitivity

Calbindin (D1I4Q) XP® Rabbit mAb recognizes endogenous levels of total calbindin protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human calbindin protein.

Background

Calcium-binding proteins of different subfamilies regulate the second messenger calcium. Calbindin, calmodulin, S-100, parvalbumin, and troponin C are members of the low molecular weight calcium-binding protein family (1). Calbindin is expressed in discrete neuronal populations within the CNS and is thought to act as an intracellular calcium buffering protein. Most Purkinje cells express calbindin, which is expressed when neurons start to migrate and differentiate. In contrast, other calcium buffering proteins, such as parvalbumin, are expressed later during development and in parallel with increasing neuronal activity (2).
For Research Use Only. Not For Use In Diagnostic Procedures.
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