Calmodulin (D1F7J) Rabbit mAb #35944

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	17
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Calmodulin (D1F7J) Rabbit mAb recognizes endogenous levels of total calmodulin protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human calmodulin protein.

Background

Calmodulin is a ubiquitously expressed small protein mediating many cellular effects such as short-term and long-term memory, nerve growth, inflammation, apoptosis, muscle contraction and intracellular movement (1). Upon binding of four Ca²⁺ ions, calmodulin undergoes conformational changes, allowing this complex to bind to and activate many enzymes including protein kinases, protein phosphatases, ion channels, Ca²⁺ pumps, nitric oxide synthase, inositol triphosphate kinase, and cyclic nucleotide phosphodiesterase (2,3). Since calmodulin binds Ca²⁺ in a cooperative fashion, small changes in cytosolic Ca²⁺ levels lead to large changes in the level of active calmodulin and its target proteins (4).

Database Links

UniProt ID: P0DP23

Entrez-Gene Id: 801

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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