Render Target: STATIC
Render Timestamp: 2024-11-22T11:40:32.633Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-08-01 15:23:59.467
Product last modified at: 2024-08-20T12:15:21.690Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

CaMKI-δ Antibody #3365

Filter:
  • WB

    Supporting Data

    REACTIVITY H R
    SENSITIVITY Endogenous
    MW (kDa) 43
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CaMKI-δ Antibody detects endogenous levels of total CamKI-δ protein. This antibody may detect other isoforms of CaMKI.

    Species Reactivity:

    Human, Rat

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Mouse

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human CaMKI-δ. Antibodies are purified by peptide affinity chromatography.

    Background

    The Ca2+/calmodulin-dependent kinase (CaMK) family, which is activated in response to elevation of intracellular Ca2+, includes CaMKI, CaMKII, CaMKIV, and CaMK-kinases (CaMKKs) (1,2). CaMKI is a downstream substrate of CaMKK and has 4 isoforms: CaMKI-α, CaMKI-β, CaMKI-γ, and CaMKI-δ. CaMKI is present in most cell types and may be involved in cellular functions, including transcription, cytoskeletal organization, axonal growth cone motility, and long-term potentiation in neurons (3-6). CaMKII is also ubiquitously expressed in most cell types. While muscular CaMKII has been linked to activation of mitochondrial biogenesis in muscle hypertrophy response, neuronal CaMKII regulates important neuronal functions, including neurotransmitter synthesis, neurotransmitter release, modulation of ion channel activity, cellular transport, cell morphology and neurite extension, synaptic plasticity, learning and memory, and gene expression (7). Like CaMKI, CaMKIV is also a substrate of CaMKKs and is primarily restricted to the nucleus of neurons. CaMKIV regulates gene transcription in neurons through phosphorylation of transcription factors such as CREB and is required for fear memory (8).

    CaMKI-δ translocates to the nucleus upon intracellular Ca2+ influx and is activated through phosphorylation of Thr180 by CaMKK (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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