Name: CaMKII-α Antibody
Price: 302.00 USD
Availability: InStock

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WB

Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	50
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CaMKII-α Antibody detects endogenous levels of total CaMKII-α. The peptide sequence used as the antigen is not conserved in CaMKII-β, -γ and -δ.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr436 of human CaMKII-α. . Antibodies are purified by protein A and peptide affinity chromatography.

Background

CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca²⁺/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4).

Database Links

UniProt ID: Q9UQM7

Entrez-Gene Id: 815

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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CaMKII-α Antibody #3357