Render Target: STATIC
Render Timestamp: 2024-11-22T10:53:52.374Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-08-01 15:23:25.524
Product last modified at: 2024-11-07T12:00:15.915Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

CaMKIV Antibody #4032

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 58, 60: human. 61, 63: rat and mouse.
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CaMKIV Antibody detects total levels of endogenous CaMKIV.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide around amino acid 282-300 of human CaMKIV. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    CaMKIV is an important member of calcium/calmodulin-activated kinases. CaMKIV signaling has been related to long-term neural potentiation and memory (1,2), as well as T-cell receptor signaling (3). CaMKIV has catalytic and regulatory domains. The Ca2+/calmodulin-dependent CaMKK phosphorylates CaMKIV, releasing the autoinhibitory effect and thus activating the kinase (4-6). The activated CaMKIV further autophosphorylates itself at Thr196 (or Thr200 in human) to render the kinase constitutively active (5). The threonine phosphorylation state of CaMKIV can be downregulated by PP2A dephosphorylation.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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