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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. Cancer-associated Growth Factor Antibody Sampler Kit

Cancer-associated Growth Factor Antibody Sampler Kit #17027

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The Cancer-associated Growth Factor Antibody Sampler Kit provides an economical means of detecting selected growth factors that have been shown to influence tumor development. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Each antibody in the Cancer-associated Growth Factor Antibody Sampler Kit detects endogenous levels of its total target protein. VEGF-A Antibody recognizes endogenous levels of all isoforms of total VEGF-A protein. This antibody also detects a 47 kDa band of unknown identity. TGF-β (56E4) Rabbit mAb detects endogenous levels of TGF-β precursor proteins. The antibody preferentially detects TGF-β1 and TGF-β3 proteins, as determined by western blot analysis of recombinant proteins. HBEGF (E5L5T) Rabbit mAb recognizes endogenous levels of total proHBEGF and mature HBEGF protein. This antibody also detects a 58 kDa band of unknown origin. MIF (E7T1W) Rabbit mAb recognizes endogenous levels of total MIF protein. Non-specific staining by MIF (E7T1W) Rabbit mAb was observed in mouse brain by immunohistochemistry. EREG (D4O5I) Rabbit mAb recognizes endogenous levels of proepiregulin and the C-terminal propeptide of the EREG protein. It does not recognize the mature form of EREG.

    Source / Purification

    Monoclonal antibodies are produced by immunizing animals with recombinant protein specific to the mature full-length human basic FGF protein, with recombinant protein fragment specific to human mature HBEGF protein, with recombinant protein specific to the carboxy terminus of human HGF protein, with synthetic peptides corresponding to residues surrounding Ser83 of human IGF-I protein, Tyr100 of human MIF protein, and Glu155 of human EREG protein, and with synthetic peptides corresponding to a region in the carboxy terminus of TGF-β1 protein.

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptides corresponding to residues surronding His38 of human VEGFA protein and Asp200 of human angiopoietin-2 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    The tumor microenvironment (TME) is composed of a heterogenous mixture of tumor cells, blood vessels, fibroblasts, stromal cells, infiltrating immune cells, and extracellular matrix (ECM) components, whose collective interactions play important roles in tumor development (1). Cells in the TME secrete a variety of bioactive molecules, including growth factors, cytokines, ECM proteins, and proteases (e.g., MMPs), many of which play critical roles in regulating growth and development of the tumor (2,3). Growth factors play particularly important roles in the TME, serving as cellular messengers that trigger activation or suppression of signaling pathways that govern tumor development, either directly via the tumor cells, or indirectly by way of effects on the TME. Binding of growth factors to their cognate receptors leads to activation of intracellular signaling pathways, resulting in changes in the expression of target genes that regulate cell behavior. Many growth factors (e.g., IGFs, HGFs, FGFs, HBEGF, EREG) are known to promote tumor development by way of direct effects on tumor cells; other growth factors can affect tumor development indirectly, through effects in the TME that influence tumor angiogenesis (e.g., VEGFs, angiopoietins), ECM deposition (TGF-β), or immune cell signaling (e.g., TGF-β, HBEGF, MIF) (4). The diverse and complex role played by growth factors in promoting tumorigenesis makes them important therapeutic targets in oncology, while elucidating the functions of specific growth factors in the context of tumor development remains an active area of cancer research (5).

    Database Links

    UniProt ID: Q99075 , P61812 , O15123 , O14944 , P14210 , P09038 , P14174 , P05019 , P15692-1 , P01137 , P10600


    Entrez-Gene Id: 1839 , 7042 , 285 , 2069 , 3082 , 2247 , 4282 , 3479 , 7422 , 7040 , 7043


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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