Render Target: STATIC
Render Timestamp: 2024-11-22T12:09:30.032Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-04-05 20:44:22.569
Product last modified at: 2024-11-11T20:00:10.532Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

CAPAM/PCIF1 Antibody #98085

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 85
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CAPAM/PCIF1 Antibody recognizes endogenous levels of total CAPAM/PCIF1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro229 of human CAPAM/PCIF1 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Phosphorylated CTD Interacting Factor 1 (PCIF1), also known as CAPAM, is a WW domain-containing protein that was initially discovered to interact with phosphorylated RNA Polymerase II, where it inhibits CTD phosphatase activity of SCP1 to negatively affect gene expression (1,2). CAPAM/PCIF1 has shown to be the methyltransferase responsible for methylating adenosine at the second position of mRNAs, promoting their translation (3,4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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