Carboxymethyl/Carboxyethyl Lysine (E5C5H) Rabbit mAb #44358

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Supporting Data

REACTIVITY	All
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Carboxymethyl/Carboxyethyl Lysine (E5C5H) Rabbit mAb recognizes a broad range of carboxymethyl lysine (CML) and carboxyethyl lysine (CEL) containing proteins and peptides. This antibody does not cross-react with free CML or CEL and does not cross-react with proteins or peptides containing acetylated, succinylated, butyrylated, glutarylated, malonylated, or propionylated lysines.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing CML/CEL flanked by degenerate amino acids at positions N- and C-terminal to the modified lysine.

Background

Carboxymethyl lysine (CML) and carboxyethyl lysine (CEL) are two protein post-translational modifications that arise from non-enzymatic pathways and represent examples of advanced glycation end products (AGEs) (1). The association between CML/CEL levels with glucose and oxidant stress levels underlies the use of these modifications as biomarkers for various disease conditions, including diabetes and osteoporosis (2). The Maillard reaction between monosaccharides and lysines can form a Schiff base intermediate that rearranges to become CML/CEL. In addition, fragmentation of the Schiff base intermediate and oxidation of amino acids and polyunsaturated fatty acids produce reactive species, such as glyoxal and methylglyoxal, which react with lysine to form CML/CEL. While CML/CEL exists as free and protein-incorporated forms, in vitro treatment with protein-incorporated CML/CEL in particular induces diverse effects, including reduced serotonin release in gastric cells, impaired calcium homeostasis in cardiac cells, and increased reactive oxygen intermediates in endothelial cells (3-5). These processes depend on receptor for AGE (RAGE) signaling.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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