Render Target: STATIC
Render Timestamp: 2024-10-11T10:23:03.142Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-20 06:19:38.960
Product last modified at: 2024-09-13T07:02:04.020Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Caspase-10 Antibody #9752

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  • WB
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Inquiry Info. # 9752

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    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 63 to 66
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Caspase-10 Antibody detects endogenous levels of full length caspase-10 and its various isoforms. The antibody does not cross-react with other caspases.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding aspartic acid 219 of human caspase-10. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Caspase-10 is a DED (death effector domain)-containing caspase and functions as an initiator caspase in Fas/TNF induced apoptosis (1). Four isoforms of caspase-10 have been identified: caspase-10a (Mch4), caspase-10b (FLICE2), caspase-10c and caspase-10d. They have the same prodomain but different mature large and small subdomains (2-4). Upon death ligand-receptor binding, caspase-10 is coupled to the multimeric Fas/TNF receptor complex via DED/FADD adaptor interaction (1-4). This complex processes procaspase-10 into a large active fragment and a small fragment. Cleaved caspase-10 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, leading to apoptosis (3-6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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