Filter:

WB

Inquiry Info. # 2202

Please see our recommended alternatives.

Supporting Data

REACTIVITY	M
SENSITIVITY	Endogenous
MW (kDa)	42, 55
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (55 kDa) and its cleaved product (42 kDa). The antibody does not cross-react with other caspases.

Species Reactivity:

Mouse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 158 of mouse caspase-12. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER stress-induced apoptosis, such as high calcium concentration, low oxygen, and low glucose levels (1,2). One of the mechanisms for caspase-12 activation is related to calpain-mediated cleavage at T132 and K158, both of which are located at the amino-terminal region of caspase-12 (2,3). Caspase-12 also has a putative caspase cleavage site located at the carboxy-terminal region of the protein (3). In cortical neurons, caspase-12 is involved at least partially in the amyloid-beta neurotoxicity process (1).

Database Links

UniProt ID: O08736

Entrez-Gene Id: 12364

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit our Trademark Information page.

Caspase-12 Antibody #2202